Neutrophil-fibroblast crosstalk drives immunofibrosis in Crohn's disease through IFNα pathway.

ABSTRACT:

Introduction

Crohn's disease (CD) is characterized by chronic inflammation and intestinal fibrosis leading to lifelong complications. However, the disease pathogenesis remains elusive, and the therapeutic options are limited. Here, we investigated the interaction between neutrophils and intestinal fibroblasts in the development of CD immunofibrosis, a disease mechanism predisposing to inflammatory and fibrotic complications.

Methods

Peripheral neutrophils, enriched neutrophil extracellular traps (eNETs), serum, primary intestinal fibroblasts (PIFs) and intestinal biopsies from CD, ulcerative colitis (UC) patients, and healthy individuals (HI), were studied. Transcriptome analysis of neutrophils, multi-cytokine profiling and cell-based functional assays at mRNA/protein level

SUBMITTER: Gavriilidis E 

PROVIDER: S-EPMC11427415 | biostudies-literature | 2024

REPOSITORIES: biostudies-literature