Characterization and Statistical Optimization of Enterobatin Synthesized by Escherichia coli OQ866153.

ABSTRACT: Microorganisms produce siderophores, which are secondary metabolites with a high affinity for iron. Siderophores have received significant attention due to their diverse applications in ecological and clinical research. In this study, siderophores production by Escherichia coli OQ866153 was optimized using two-stage statistical approach involving Plackett-Burman design (PBD) and response surface methodology (RSM) using central composite design (CCD). Out of 23 variables, succinate, tryptophan, Na₂HPO₄, CaCl₂, agitation, and KH₂PO₄ were found to have the most significant effect on siderophores production in the first optimization stage with the highest SU% of 43.67%. In the second stage, RSM using CCD was utilized, and the optimal condi

SUBMITTER: Khazaal MT 

PROVIDER: S-EPMC11427530 | biostudies-literature | 2024 Oct

REPOSITORIES: biostudies-literature