Activation of Piezo1 by intracranial hypertension induced neuronal apoptosis via activating hippo pathway.

ABSTRACT:

Aim

Most of the subarachnoid hemorrhage (SAH) patients experienced the symptom of severe headache caused by intracranial hypertension. Piezo1 is a mechanosensitive ion channel protein. This study aimed to investigate the effect of Piezo1 on neurons in response to intracranial hypertension.

Methods

The SAH rat model was performed by the modified endovascular perforation method. Piezo1 inhibitor GsMTx4 was administered intraperitoneally after SAH induction. To investigate the underlying mechanism, the selective Piezo1 agonist Yoda1, Piezo1 shRNA, and MY-875 were administered via intracerebroventricular injection before SAH induction. In vitro, we designed a pressurizing device to exclusively explore the effect of Piezo1 activation on primary neurons. Neurons were pretreated wi

SUBMITTER: Zeng J 

PROVIDER: S-EPMC11427798 | biostudies-literature | 2024 Sep

REPOSITORIES: biostudies-literature