Characteristics of PD-1⁺CD4⁺ T cells in peripheral blood and synovium of rheumatoid arthritis patients.

ABSTRACT:

Objectives

PD-1 plays a crucial role in the immune dysregulation of rheumatoid arthritis (RA), but the specific characteristics of PD-1⁺CD4⁺ T cells remain unclear and require further investigation.

Methods

Circulating PD-1⁺CD4⁺ T cells from RA patients were analysed using flow cytometry. Plasma levels of soluble PD-1 (sPD-1) were measured using enzyme-linked immunosorbent assay (ELISA). Single-cell RNA sequence data from peripheral blood mononuclear cells (PBMCs) and synovial tissue of patients were obtained from the GEO and the ImmPort databases. Bioinformatics analyses were performed in the R studio to characterise PD-1⁺CD4⁺ T cells. Expression of CCR7, KLF2 and IL32 in PD-1⁺CD4⁺ T cel

SUBMITTER: Chen YJ 

PROVIDER: S-EPMC11427813 | biostudies-literature | 2024

REPOSITORIES: biostudies-literature