UV/Visible Diffusion-Ordered Spectroscopy: A Simultaneous Probe of Molecular Size and Electronic Absorption.

ABSTRACT: Based on concepts from nuclear magnetic resonance, we have developed UV/vis diffusion-ordered spectroscopy, which simultaneously probes the size and electronic absorption spectrum of molecules and particles. We use simple flow technology to create a step-function concentration profile inside an optical sample cell, and by measuring the time-dependent absorption spectrum in an initially solvent-filled part of the sample volume, we obtain the diffusion coefficients and UV/vis spectra of the species present in the sample solution. From these data, we construct a two-dimensional spectrum with absorption wavelength on one axis and diffusion coefficient (or equivalently, size) on the other, in which the UV/vis spectrum of a mixture with different molecular sizes is separated into the spectra of

SUBMITTER: Giubertoni G 

PROVIDER: S-EPMC11428122 | biostudies-literature | 2024 Sep

REPOSITORIES: biostudies-literature