CD9 promotes TβR2-TβR1 association driving the transition of human dermal fibroblasts to myofibroblast under hypoxia.

ABSTRACT:

Background

During wound healing, fibroblast to myofibroblast transition is required for wound contraction and remodeling. While hypoxia is an important biophysical factor in wound microenvironment, the exact regulatory mechanism underlying hypoxia and fibroblast-to-myofibroblast transition remains unclear. We previously found that tetraspanin CD9 plays an important role in oxygen sensing and wound healing. Herein, we investigated the effects of physiological hypoxia on fibroblast-to-myofibroblast transition and the biological function and mechanism of CD9 in it.

Methods

Human skin fibroblasts (HSF) and mouse dermis wounds model were established under physiological hypoxia (2% O₂). The cell viability and contractility of HSF under hypoxia were evaluated by CCK8 and

SUBMITTER: Huang W 

PROVIDER: S-EPMC11428569 | biostudies-literature | 2024 Sep

REPOSITORIES: biostudies-literature