The interaction between neutrophils and atrial myocytes in the occurrence and development of atrial fibrillation.

ABSTRACT:

Background

Atrial fibrillation (AF) is one of the most prevalent sustained cardiac arrhythmias, strongly associated with neutrophils. However, the underlying mechanism remain unclear. This study aims to explore the interaction between neutrophils and atrial myocytes in the pathogenesis of AF.

Methods

Patch-clamp was employed to record the action potential duration (APD) and ion channels in HL-1 cells. Flow cytometry was used to assess the differentiation of neutrophils. The mRNA and protein levels of CACNA1C, CACNA2D, and CACNB2 in HL-1 cells were detected.

Results

High-frequency electrical stimulation resulted in a shortening of the APD in HL-1 cells. Flow cytometry demonstrated that neutrophils were polarized into N1 phenotype when cultured with stimulated HL-1 cell

SUBMITTER: Li T 

PROVIDER: S-EPMC11428612 | biostudies-literature | 2024 Sep

REPOSITORIES: biostudies-literature