Indoxyl sulfate induces retinal microvascular injury via COX-2/PGE₂ activation in diabetic retinopathy.

ABSTRACT:

Background

Diabetic retinopathy (DR), the principal cause of acquired blindness among the working-age population, is the most frequent microvascular complication of diabetes. Although metabolic disorders are hypothesized to play a role in its pathogenesis, the underlying mechanism remains largely elusive.

Methods

To elucidate the mechanism, we initially compared metabolite profiles of vitreous fluid between 23 patients with DR and 12 non-diabetic controls using liquid chromatography/tandem mass spectrometry, identifying the distinct metabolite indoxyl sulfate (IS). Subsequently, streptozotocin (STZ)-induced diabetic and IS-injected rat models were established to examine the effects of IS on retinal microvasculature. RNA sequencing was conducted to identify potential regulato

SUBMITTER: Zhou L 

PROVIDER: S-EPMC11428830 | biostudies-literature | 2024 Sep

REPOSITORIES: biostudies-literature