Tetraspanin 3 promotes NSCLC cell proliferation via regulation of β1 integrin intracellular recycling.

ABSTRACT:

Background

The involvement of tetraspanins in cancer development has been widely implicated. In this study, the function and molecular mechanisms of tetraspanin 3 (TSPAN3) in non-small cell lung cancer (NSCLC) cells were explored.

Methods

Tissue samples from patients diagnosed with NSCLC were analyzed by immunohistochemistry, western blotting, and real-time polymerase chain reaction (PCR) to indicate the involvement of TSPAN3 in cancer progression. In the meantime, we also performed exhaustive mechanistic studies using A549 and H460 cells in vitro through a variety of methods including western blotting, real-time PCR, immunofluorescent staining, coimmunoprecipitation, cell proliferation assay, and nocodazole (NZ) washout assay. Proper statistical analysis was implemented whe

SUBMITTER: Zhang Y 

PROVIDER: S-EPMC11428915 | biostudies-literature | 2024 Sep

REPOSITORIES: biostudies-literature