Spatial microenvironments tune immune response dynamics in the <i>Drosophila</i> larval fat body.

ABSTRACT: Immune responses in tissues display intricate patterns of gene expression that vary across space and time. While such patterns have been increasingly linked to disease outcomes, the mechanisms that generate them and the logic behind them remain poorly understood. As a tractable model of spatial immune responses, we investigated heterogeneous expression of antimicrobial peptides in the larval fly fat body, an organ functionally analogous to the liver. To capture the dynamics of immune response across the full tissue at single-cell resolution, we established live light sheet fluorescence microscopy of whole larvae. We discovered that expression of antimicrobial peptides occurs in a reproducible spatial pattern, with enhanced expression in the anterior and posterior lobes of the fat body. Thi

SUBMITTER: Schlomann BH 

PROVIDER: S-EPMC11429692 | biostudies-literature | 2024 Sep

REPOSITORIES: biostudies-literature