Quantitative Comparison of Monomeric StayGold Variants Using Protein Nanocages in Living Cells.

ABSTRACT: To standardize comparison of fluorescent proteins and independently determine which monomeric StayGold variant is best for live microscopy, we analyzed fluorescent protein tagged I3-01 peptides that self-assemble into stable sixty subunit dodecahedrons inside live cells. We find mStayGold is 3-fold brighter and 3-fold more photostable compared with EGFP and superior to other monomeric variants in mammalian cytoplasm. In addition, analysis of intracellular nanocage diffusion confirms the monomeric nature of mStayGold.

SUBMITTER: Viola G 

PROVIDER: S-EPMC11429717 | biostudies-literature | 2024 Sep

REPOSITORIES: biostudies-literature