Integrating collecting systems in kidney organoids through fusion of distal nephron to ureteric bud.

ABSTRACT: The kidney maintains homeostasis through an array of parallel nephrons, which all originate in development as isolated epithelial structures that later fuse through their distal poles to a system of collecting ducts (CD). This connection is required to generate functional nephrons by providing a pathway for excretion of metabolic waste and byproducts. Currently, methods for differentiating human pluripotent stem cells into kidney organoids generate nephrons that lack CDs and instead terminate as blind-ended tubules. Here we describe a developmentally inspired system that addresses this deficiency through assembly of induced nephrogenic mesenchyme with ureteric bud (UB) tissues, the embryonic building blocks of the kidney's collecting system. The UB progenitors grow and develop into a netwo

SUBMITTER: Shi M 

PROVIDER: S-EPMC11429897 | biostudies-literature | 2024 Sep

REPOSITORIES: biostudies-literature