The Precise Basecalling of Short-Read Nanopore Sequencing.

ABSTRACT: The nanopore sequencing of short sequences, whose lengths are typically less than 0.3kb therefore comparable with Illumina sequencing techniques, has recently gained wide attention. Here, we design a scheme for training nanopore basecallers that are specialized for short biomolecules. With bioengineered RNA (BioRNA) molecules as examples, we demonstrate the superior accuracy of basecallers trained by our scheme.

SUBMITTER: Wang Z 

PROVIDER: S-EPMC11429927 | biostudies-literature | 2024 Sep

REPOSITORIES: biostudies-literature