Multiplexed spatial mapping of chromatin features, transcriptome, and proteins in tissues.

ABSTRACT: The phenotypic and functional states of a cell are modulated by a complex interactive molecular hierarchy of multiple omics layers, involving the genome, epigenome, transcriptome, proteome, and metabolome. Spatial omics approaches have enabled the capture of information from different molecular layers directly in the tissue context. However, current technologies are limited to map one to two modalities at the same time, providing an incomplete representation of cellular identity. Such data is inadequate to fully understand complex biological systems and their underlying regulatory mechanisms. Here we present spatial-Mux-seq, a multi-modal spatial technology that allows simultaneous profiling of five different modalities, including genome-wide profiles of two histone modifications and open

SUBMITTER: Guo P 

PROVIDER: S-EPMC11429933 | biostudies-literature | 2024 Sep

REPOSITORIES: biostudies-literature