TIRTL-seq: Deep, quantitative, and affordable paired TCR repertoire sequencing.

ABSTRACT: ɑ/β T cells are key players in adaptive immunity. The specificity of T cells is determined by the sequences of the hypervariable T cell receptor (TCR) ɑ and β chains. Although bulk TCR sequencing offers a cost-effective approach for in-depth TCR repertoire profiling, it does not provide chain pairings, which are essential for determining T cell specificity. In contrast, single-cell TCR sequencing technologies produce paired chain data, but are limited in throughput to thousands of cells and are cost-prohibitive for cohort-scale studies. Here, we present TIRTL-seq (Throughput-Intensive Rapid TCR Library sequencing), a novel approach that generates ready-to-sequence TCR libraries from live cells in less than 7 hours. The protocol is optimized

SUBMITTER: Pogorelyy MV 

PROVIDER: S-EPMC11430070 | biostudies-literature | 2024 Oct

REPOSITORIES: biostudies-literature