A Nanobody of PEDV S1 Protein: Screening and Expression in Escherichia coli.

ABSTRACT: Porcine epidemic diarrhea virus (PEDV) has caused significant economic losses to the pig farming industry in various countries for a long time. Currently, there are no highly effective preventive or control measures available. Research into the pathogenic mechanism of PEDV has shown that it primarily causes infection by binding the S protein to the CD13 (APN) receptor on the membrane of porcine intestinal epithelial cells. The S1 region contains three neutralization epitopes and multiple receptor-binding domains, which are closely related to viral antigenicity and ad-sorption invasion. Nanobodies are a type of single-domain antibody that have been discovered in recent years. They can be expressed on a large scale through prokaryotic expression systems, which makes them cost-effective, stab

SUBMITTER: Hao Z 

PROVIDER: S-EPMC11430113 | biostudies-literature | 2024 Sep

REPOSITORIES: biostudies-literature