Screening of Anti-Prion Compounds Using the Protein Misfolding Cyclic Amplification Technology.

ABSTRACT: Prion diseases are 100% fatal infectious neurodegenerative diseases affecting the brains of humans and other mammals. The disease is caused by the formation and replication of prions, composed exclusively of the misfolded prion protein (PrP^Sc). We invented and developed the protein misfolding cyclic amplification (PMCA) technology for in vitro prion replication, which allow us to replicate the infectious agent and it is commonly used for ultra-sensitive prion detection in biological fluids, tissues and environmental samples. In this article, we studied whether PMCA can be used to screen for chemical compounds that block prion replication. A small set of compounds previously shown to have anti-prion activity in various systems, mostly using cells infected with murine prions, was

SUBMITTER: Pritzkow S 

PROVIDER: S-EPMC11430292 | biostudies-literature | 2024 Sep

REPOSITORIES: biostudies-literature