Biochemical Characterisation of the Short Isoform of Histone N-Terminal Acetyltransferase NAA40.

ABSTRACT: N-alpha-acetyltransferase 40 (NAA40) is an evolutionarily conserved N-terminal acetyltransferase (NAT) linked to oncogenesis and chemoresistance. A recent study reported the generation of a second, shorter NAA40 isoform (NAA40^S) through alternative translation, which we proceeded to further characterise. Notably, recombinant NAA40^S had a greater in vitro enzymatic activity and affinity towards its histone H2A/H4 substrates compared to full-length NAA40 (NAA40^L). Within cells, NAA40^S was enzymatically active, based on its ability to suppress the H2A/H4S1Ph antagonistic mark in CRISPR-generated NAA40 knockout cells. Finally, we show that in addition to alternative translation, the NAA40^S isoform could be derived from a primate and

SUBMITTER: Klavaris A 

PROVIDER: S-EPMC11430322 | biostudies-literature | 2024 Sep

REPOSITORIES: biostudies-literature