Monocyte Single-Cell Multimodal Profiling in Cardiovascular Disease Risk States.

ABSTRACT:

Background

Monocytes are a critical innate immune system cell type that serves homeostatic and immunoregulatory functions. They have been identified historically by the cell surface expression of CD14 and CD16. However, recent single-cell studies have revealed that they are much more heterogeneous than previously realized.

Methods

We utilized cellular indexing of transcriptomes and epitopes by sequencing (CITE-seq) and single-cell RNA sequencing to describe the comprehensive transcriptional and phenotypic landscape of 437 126 monocytes.

Results

This high-dimensional multimodal approach identified vast phenotypic diversity and functionally distinct subsets, including IFN-responsive, MHCII^hi (major histocompatibility complex class II), monocyte-platelet aggre

SUBMITTER: Bashore AC 

PROVIDER: S-EPMC11430373 | biostudies-literature | 2024 Aug

REPOSITORIES: biostudies-literature