Exploration into Galectin-3 Driven Endocytosis and Lattices.

ABSTRACT: Essentially all plasma membrane proteins are glycosylated, and their activity is regulated by tuning their cell surface dynamics. This is achieved by glycan-binding proteins of the galectin family that either retain glycoproteins within lattices or drive their endocytic uptake via the clathrin-independent glycolipid-lectin (GL-Lect) mechanism. Here, we have used immunofluorescence-based assays to analyze how lattice and GL-Lect mechanisms affect the internalization of the cell adhesion and migration glycoprotein α₅β₁ integrin. In retinal pigment epithelial (RPE-1) cells, internalized α₅β₁ integrin is found in small peripheral endosomes under unperturbed conditions. Pharmacological compounds were used to competitively inhibit one of the galectin f

SUBMITTER: Shafaq-Zadah M 

PROVIDER: S-EPMC11430376 | biostudies-literature | 2024 Sep

REPOSITORIES: biostudies-literature