A Novel Dhillonvirus Phage against Escherichia coli Bearing a Unique Gene of Intergeneric Origin.

ABSTRACT: Antibiotics resistance is expanding amongst pathogenic bacteria. Phage therapy is a revived concept for targeting bacteria with multiple antibiotics resistances. In the present study, we isolated and characterized a novel phage from hospital treatment plant input, using Escherichia coli (E. coli) as host bacterium. Phage lytic activity was detected by using soft agar assay. Whole-genome sequencing of the phage was performed by using Next-Generation Sequencing (NGS). Host range was determined using other species of bacteria and representative genogroups of E. coli. Whole-genome sequencing of the phage revealed that Escherichia phage Ioannina is a novel phage within the Dhillonvirus genus, but significantly diverged from other Dhillonviruses. Its genome is a 45,27

SUBMITTER: Vasileiadis A 

PROVIDER: S-EPMC11430396 | biostudies-literature | 2024 Aug

REPOSITORIES: biostudies-literature