Development of a Modular miRNA-Responsive Biosensor for Organ-Specific Evaluation of Liver Injury.

ABSTRACT: MicroRNAs (miRNAs) are increasingly being considered essential diagnostic biomarkers and therapeutic targets for multiple diseases. In recent years, researchers have emphasized the need to develop probes that can harness extracellular miRNAs as input signals for disease diagnostics. In this study, we introduce a novel miRNA-responsive biosensor (miR-RBS) designed to achieve highly sensitive and specific detection of miRNAs, with a particular focus on targeted organ-specific visualization. The miR-RBS employs a Y-structured triple-stranded DNA probe (Y-TSDP) that exhibits a fluorescence-quenched state under normal physiological conditions. The probe switches to an activated state with fluorescence signals in the presence of high miRNA concentrations, enabling rapid and accurate disease repo

SUBMITTER: Zhang X 

PROVIDER: S-EPMC11430419 | biostudies-literature | 2024 Sep

REPOSITORIES: biostudies-literature