An Enhanced Retroviral Vector for Efficient Genetic Manipulation and Selection in Mammalian Cells.

ABSTRACT: Introducing genetic material into hard-to-transfect mammalian cell lines and primary cells is often best achieved through retroviral infection. An ideal retroviral vector should offer a compact, selectable, and screenable marker while maximizing transgene delivery capacity. However, a previously published retroviral vector featuring an EGFP/Puromycin fusion protein failed to meet these criteria in our experiments. We encountered issues such as low infection efficiency, weak EGFP fluorescence, and selection against infected cells. To address these shortcomings, we developed a novel retroviral vector based on the Moloney murine leukemia virus. This vector includes a compact bifunctional EGFP and Puromycin resistance cassette connected by a 2A peptide. Our extensively tested vector demonstrat

SUBMITTER: Triller J 

PROVIDER: S-EPMC11430422 | biostudies-literature | 2024 Sep

REPOSITORIES: biostudies-literature