Optical Interferometric Device for Rapid and Specific Detection of Biological Cells.

ABSTRACT: Here, we report a rapid and accurate optical method for detecting cells from liquid samples in a label-free manner. The working principle of the method is based on the interference of parts of a conical laser beam, coming from a single-mode optical fiber directly, and reflected from a flat glass surface. The glass is functionalized by antibodies against the cells to be detected from the liquid sample. Cells bound to that surface modify the reflected beam, and hence, change the resulting interference pattern, too. By registering and interpreting the variation in the image, the presence of cells from the sample can be detected. As for a demonstration, cell suspensions from a U937 cell line were used in glass chambers functionalized by antibodies (TMG6-5 (mIgG1)) to which the cells specifical

SUBMITTER: Valkai S 

PROVIDER: S-EPMC11430435 | biostudies-literature | 2024 Aug

REPOSITORIES: biostudies-literature