Establishment of an Agrobacterium tumefaciens-Mediated Transformation System for Hirsutella sinensis.

ABSTRACT: Ophiocordyceps sinensis (Berk.) is a complex is formed by Hepialidae larvae and Hirsutella sinensis. Infestation by H. sinensis, interaction with host larvae, and fruiting body development are three crucial processes affecting the formation of O. sinensis. However, research on the molecular mechanism of O. sinensis formation has been hindered by the lack of effective genetic transformation protocols. Therefore, Agrobacterium tumefaciens-mediated transformation (ATMT) was adopted to genetically transform two H. sinensis strains and optimize the transformation conditions. The results revealed that the most suitable Agrobacterium strain for H. sinensis transformation was AGL1, and that the surfactant Triton X-100 could also

SUBMITTER: Wu L 

PROVIDER: S-EPMC11430471 | biostudies-literature | 2024 Sep

REPOSITORIES: biostudies-literature