Combined assembloid modeling and 3D whole-organ mapping captures the microanatomy and function of the human fallopian tube.

ABSTRACT: The fallopian tubes play key roles in processes from pregnancy to ovarian cancer where three-dimensional (3D) cellular and extracellular interactions are important to their pathophysiology. Here, we develop a 3D multicompartment assembloid model of the fallopian tube that molecularly, functionally, and architecturally resembles the organ. Global label-free proteomics, innovative assays capturing physiological functions of the fallopian tube (i.e., oocyte transport), and whole-organ single-cell resolution mapping are used to validate these assembloids through a multifaceted platform with direct comparisons to fallopian tube tissue. These techniques converge at a unique combination of assembloid parameters with the highest similarity to the reference fallopian tube. This work establishes (i)

SUBMITTER: Crawford AJ 

PROVIDER: S-EPMC11430475 | biostudies-literature | 2024 Sep

REPOSITORIES: biostudies-literature