3D Modeling: Insights into the Metabolic Reprogramming of Cholangiocarcinoma Cells.

ABSTRACT: Developing accurate in vitro models that replicate the in vivo tumor environment is essential for advancing cancer research and therapeutic development. Traditional 2D cell cultures often fail to capture the complex structural and functional heterogeneity of tumors, limiting the translational relevance of findings. In contrast, 3D culture systems, such as spheroids, provide a more physiologically relevant context by replicating key aspects of the tumor microenvironment. This study aimed to compare the metabolism of three intrahepatic cholangiocarcinoma cell lines in 2D and 3D cultures to identify metabolic shifts associated with spheroid formation. Cells were cultured in 2D on adhesion plates and in 3D using ultra-low attachment plates. Metabolic exchange rates were measured using NMR, and

SUBMITTER: Ciufolini G 

PROVIDER: S-EPMC11430555 | biostudies-literature | 2024 Sep

REPOSITORIES: biostudies-literature