Ultrasensitive Electrochemical Biosensors Based on Allosteric Transcription Factors (aTFs) for Pb²⁺ Detection.

ABSTRACT: Exposure to Pb²⁺ in the environment, especially in water, poses a significant threat to human health and urgently necessitates the development of highly sensitive Pb²⁺ detection methods. In this study, we have integrated the high sensitivity of electrochemical techniques with allosteric transcription factors (aTFs) to develop an innovative electrochemical biosensing platform. This biosensors leverage the specific binding and dissociation of DNA to the aTFs (PbrR) on electrode surfaces to detect Pb²⁺. Under the optimal conditions, the platform has a broad linear detection range from 1 pM to 10 nM and an exceptionally low detection threshold of 1 pM, coupled with excellent selectivity for Pb²⁺. Notably, the biosensor demonstrates regenerative capab

SUBMITTER: Yu N 

PROVIDER: S-EPMC11430688 | biostudies-literature | 2024 Sep

REPOSITORIES: biostudies-literature