Impacts of Nucleosome Positioning Elements and Pre-Assembled Chromatin States on Expression and Retention of Transgenes.

ABSTRACT:

Background/objectives

Transgene applications, ranging from gene therapy to the development of stable cell lines and organisms, rely on maintaining the expression of transgenes. To date, the use of plasmid-based transgenes has been limited by the loss of their expression shortly after their delivery into the target cells. The short-lived expression of plasmid-based transgenes has been largely attributed to host-cell-mediated degradation and/or silencing of transgenes. The development of chromatin-based strategies for gene delivery has the potential to facilitate defining the requirements for establishing epigenetic states and to enhance transgene expression for numerous applications.

Methods

To assess the impact of "priming" plasmid-based transgenes to adopt accessible chroma

SUBMITTER: Kwizera R 

PROVIDER: S-EPMC11431089 | biostudies-literature | 2024 Sep

REPOSITORIES: biostudies-literature