Benchtop IR Imaging of Live Cells: Monitoring the Total Mass of Biomolecules in Single Cells.

ABSTRACT: Absolute quantity imaging of biomolecules on a single cell level is critical for measurement assurance in biosciences and bioindustries. While infrared (IR) transmission microscopy is a powerful label-free imaging modality capable of chemical quantification, its applicability to hydrated biological samples remains challenging due to the strong IR absorption by water. Traditional IR imaging of hydrated cells relies on powerful light sources, such as synchrotrons, to mitigate the light absorption by water. However, we overcome this challenge by applying a solvent absorption compensation (SAC) technique to a home-built benchtop IR microscope based on an external-cavity quantum cascade laser. SAC-IR microscopy adjusts the incident light using a pair of polarizers to precompensate the IR absorp

SUBMITTER: Chang YR 

PROVIDER: S-EPMC11431153 | biostudies-literature | 2024 Sep

REPOSITORIES: biostudies-literature