A Custom qPCR Assay to Simultaneously Quantify Human and Microbial DNA.

ABSTRACT: To date, studies on microbial forensics have focused mainly on sequence analysis and generally do not include information on the quantification of and comparison between the human and bacterial DNA present in forensic samples. Knowing the amount of each type of DNA can be important for determining when and how best to employ bacterial DNA analysis, especially when there is insufficient human DNA for successful short tandem repeat (STR) typing. The goal of this work was to develop a quantitative PCR (qPCR) assay that simultaneously quantifies human and bacterial DNA that would be simple and cost-effective for laboratories to implement. Through a reproducibility study and several small-scale experiments, the reliability of a custom qPCR assay was established. A reproducibility study illustra

SUBMITTER: Foster M 

PROVIDER: S-EPMC11431276 | biostudies-literature | 2024 Aug

REPOSITORIES: biostudies-literature