Substrate-Mediated Regulation of Src Expression Drives Osteoclastogenesis Divergence.

ABSTRACT:

Background/objectives

Glass, bone, and dentin are commonly applied substrates for osteoclast cultures; however, the impact of these substrates on osteoclastogenesis remains underexplored. This study aimed to address a significant gap in understanding how different substrates influence the process of osteoclastogenesis.

Methods

RAW 264.7 cells were cultured and induced with RANKL on glass, bone, and dentin slides. Histological and molecular techniques were used to identify patterns and differences in osteoclast behavior on each substrate.

Results

Osteoclasts cultured on glass slides possessed the greatest number of nuclei and the highest expression levels of ACP5 (TRAP) and CTSK, with osteoclasts on bone and dentin slides displaying progressively lower levels. Src expr

SUBMITTER: Hu B 

PROVIDER: S-EPMC11431296 | biostudies-literature | 2024 Sep

REPOSITORIES: biostudies-literature