Effects of Temperature and Time on the Denaturation of Transforming Growth Factor Beta-1 and Cytokines from Bovine Platelet-Rich Gel Supernatants.

ABSTRACT: There is a lack of information about transforming growth factor beta-1 (TGF-β₁) and cytokines contained in pure platelet-rich plasma (P-PRP) and release from pure-platelet-rich gel supernatants (P-PRGS) might be affected by the temperature and time factors; P-PRP from 6 heifers was activated with calcium gluconate. Thereafter, P-PRG and their supernatants (P-PRGS) were maintained at -80, -20, 4, 21, and 37 °C and collected at 3, 6, 12, 24, 48, 96, 144, 192, 240, and 280 h for subsequent determination of TGF-β₁, tumor necrosis factor alfa (TNF-α), interleukin (IL)-2, and IL-6; TGF-β₁ concentrations were significantly (p < 0.05) higher in PRGS maintained at 21 and 37 °C when compared to PRGS maintained at 4, -20, and -80 °C; PRGS TNF-α concentrations w

SUBMITTER: Carmona JU 

PROVIDER: S-EPMC11431824 | biostudies-literature | 2024 Sep

REPOSITORIES: biostudies-literature