Impact of Shaking EDTA, Citrate, or MgSO₄ Tubes on Platelet Count Results.

ABSTRACT: Background: In EDTA-induced pseudothrombopenia, citrate or MgSO₄ are recommended for platelet counting. Pre-analytical conditions are poorly defined for tubes containing MgSO₄ or citrate. In this study, we analyzed the impact of agitation of these tubes on platelet counts. Methods: K2EDTA, citrate, and MgSO₄ tubes from 70 patients were gently agitated on a wheel rotating at 20 rpm. Platelets were analyzed on the Sysmex XN analyzer at different times, and the percentage of platelet deviation from T0 was assessed and compared with the desirable bias of the EFLM. Results: at 180 min in fluorescence, the relative variation of platelets after shaking is 1.17% for K2EDTA, -29.76% for citrate, and -33.18% for MgSO₄, while for unshak

SUBMITTER: Soulard M 

PROVIDER: S-EPMC11432049 | biostudies-literature | 2024 Sep

REPOSITORIES: biostudies-literature