Functional Interdependence of Anoctamins May Influence Conclusions from Overexpression Studies.

ABSTRACT: Anoctamin 6 (ANO6, TMEM16F) is a phospholipid (PL) scramblase that moves PLs between both plasma membrane (PM) leaflets and operates as an ion channel. It plays a role in development and is essential for hemostasis, bone mineralization and immune defense. However, ANO6 has also been shown to regulate cellular Ca²⁺ signaling and PM compartments, thereby controlling the expression of ion channels such as CFTR. Given these pleiotropic effects, we investigated the functional interdependence of the ubiquitous ANO6 with other commonly co-expressed anoctamins. As most expression studies on anoctamins use HEK293 human embryonic kidney cells, we compared ion currents, PL scrambling and Ca²⁺ signals induced by the overexpression of anoctamins in HEK293 wild-type parental and AN

SUBMITTER: Ousingsawat J 

PROVIDER: S-EPMC11432102 | biostudies-literature | 2024 Sep

REPOSITORIES: biostudies-literature