Optimization of Helicobacter pylori Biofilm Formation in In Vitro Conditions Mimicking Stomach.

ABSTRACT: Helicobacter pylori is one of the most common bacterial pathogens worldwide and the main etiological agent of numerous gastric diseases. The frequency of multidrug resistance of H. pylori is growing and the leading factor related to this phenomenon is its ability to form biofilm. Therefore, the establishment of a proper model to study this structure is of critical need. In response to this, the aim of this original article is to validate conditions of the optimal biofilm development of H. pylori in monoculture and co-culture with a gastric cell line in media simulating human fluids. Using a set of culture-based and microscopic techniques, we proved that simulated transcellular fluid and simulated gastric fluid, when applied in appropriate concentrations, stimulate auto

SUBMITTER: Krzyzek P 

PROVIDER: S-EPMC11432336 | biostudies-literature | 2024 Sep

REPOSITORIES: biostudies-literature