Comparative evaluation of soil DNA extraction kits for long read metagenomic sequencing.

ABSTRACT: Metagenomics has been transformative in our understanding of the diversity and function of soil microbial communities. Applying long read sequencing to whole genome shotgun metagenomics has the potential to revolutionise soil microbial ecology through improved taxonomic classification, functional characterisation and metagenome assembly. However, optimisation of robust methods for long read metagenomics of environmental samples remains undeveloped. In this study, Oxford Nanopore sequencing using samples from five commercially available soil DNA extraction kits was compared across four soil types, in order to optimise read length and reproducibility for comparative long read soil metagenomics. Average extracted DNA lengths varied considerably between kits, but longer DNA fragments did not t

SUBMITTER: Child HT 

PROVIDER: S-EPMC11432601 | biostudies-literature | 2024

REPOSITORIES: biostudies-literature