Energy Metabolism Enhance Perylenequinone Biosynthesis in <i>Shiraia</i> sp. Slf14 through Promoting Mitochondrial ROS Accumulation.

ABSTRACT: Perylenequinones (PQs) are important natural compounds that have been extensively utilized in recent years as agents for antimicrobial, anticancer, and antiviral photodynamic therapies. In this study, we investigated the molecular mechanisms regulating PQ biosynthesis by comparing Shiraia sp. Slf14 with its low PQ titer mutant, Slf14(w). The results indicated that the strain Slf14 exhibited a higher PQ yield, a more vigorous energy metabolism, and a more pronounced oxidation state compared to Slf14(w). Transcriptome analysis consistently revealed that the differences in gene expression between Slf14 and Slf14(w) are primarily associated with genes involved in redox processes and energy metabolism. Additionally, reactive oxygen species (ROS) were shown to play a crucial role in promo

SUBMITTER: Wu X 

PROVIDER: S-EPMC11432641 | biostudies-literature | 2024 Sep

REPOSITORIES: biostudies-literature