Transplanted Murine Tumours SPECT Imaging with ^99mTc Delivered with an Artificial Recombinant Protein.

ABSTRACT: ^99mTc is a well-known radionuclide that is widely used and readily available for SPECT/CT (Single-Photon Emission Computed Tomography) diagnosis. However, commercial isotope carriers are not specific enough to tumours, rapidly clear from the bloodstream, and are not safe. To overcome these limitations, we suggest immunologically compatible recombinant proteins containing a combination of metal binding sites as ^99mTc chelators and several different tumour-specific ligands for early detection of tumours. E1b protein containing metal-binding centres and tumour-specific ligands targeting integrin α_vβ₃ and nucleolin, as well as a short Cys-rich sequence, was artificially constructed. It was produced in E. coli, purified by metal-chelate chromat

SUBMITTER: Pozdniakova NV 

PROVIDER: S-EPMC11432708 | biostudies-literature | 2024 Sep

REPOSITORIES: biostudies-literature