Exploring the Role of Fibrin Gels in Enhancing Cell Migration for Vasculature Formation.

ABSTRACT: A hallmark of angiogenesis is the sprouting of endothelial cells. To replicate this event in vitro, biomaterial approaches can play an essential role in promoting cell migration. To study the capacity of a scaffold of fibrin (fibrinogen:thrombin mix) to support the movement of the endothelial cells, the migration area of spheroids formed with the HULEC cell line was measured. The cells were first allowed to form a spheroid using the hanging drop technique before being encapsulated in the fibrin gel. The cells' migration area was then measured after two days of embedding in the fibrin gel. Various conditions affecting fibrin gel polymerization, such as different concentrations of fibrinogen and thrombin, were evaluated alongside rheology, porosity, and fiber thickness analysis to understand

SUBMITTER: Moura JA 

PROVIDER: S-EPMC11432887 | biostudies-literature | 2024 Sep

REPOSITORIES: biostudies-literature