Regulation of Safracin Biosynthesis and Transport in Pseudomonas poae PMA22.

ABSTRACT: Pseudomonas poae PMA22 produces safracins, a family of compounds with potent broad-spectrum anti-bacterial and anti-tumor activities. The safracins' biosynthetic gene cluster (BGC sac) consists of 11 ORFs organized in two divergent operons (sacABCDEFGHK and sacIJ) that are controlled by P_a and P_i promoters. Contiguous to the BGC sac, we have located a gene that encodes a putative global regulator of the LysR family annotated as MexT that was originally described as a transcriptional activator of the MexEF-OprN multidrug efflux pump in Pseudomonas. Through both in vitro and in vivo experiments, we have demonstrated the involvement of the dual regulatory system MexT-MexS on the BGC sac expression acting as an activator and a rep

SUBMITTER: Hernandez Delgado JG 

PROVIDER: S-EPMC11432991 | biostudies-literature | 2024 Sep

REPOSITORIES: biostudies-literature