Calorie Restriction Decreases Competitive Fitness in Saccharomyces cerevisiae Following Heat Stress.

ABSTRACT: Experiments exposing Saccharomyces cerevisiae to glucose limitation (calorie restriction) are widely used to determine impacts on cell health as a model for aging. Using growth on plates and in liquid culture, we demonstrated that calorie restriction reduces fitness in subsequent nutrient-limited environments. Yeast grown in a calorie-restricted environment took longer to emerge from the lag phase, had an extended doubling time and had a lower percentage of culturability. Cells grown under moderate calorie restriction were able to withstand a gradual heat stress in a similar manner to cells grown without calorie restriction but fared less well with a sudden heat shock. Yeast grown under extreme calorie restriction were less fit when exposed to gradual heating or heat shock. Using RN

SUBMITTER: Hill L 

PROVIDER: S-EPMC11433872 | biostudies-literature | 2024 Sep

REPOSITORIES: biostudies-literature