Characterization of gut symbionts from wild-caught <i>Drosophila</i> and other Diptera: description of <i>Utexia brackfieldae</i> gen. nov., sp. nov., <i>Orbus sturtevantii</i> sp. nov., <i>Orbus wheelerorum</i> sp. nov, and <i>Orbus mooreae</i> sp. nov.

ABSTRACT: Non-culture based surveys show that the bacterial family Orbaceae is widespread in guts of insects, including wild Drosophila. Relatively few isolates have been described, and none has been described from Drosophila. We present the isolation and characterization of five strains of Orbaceae from wild-caught flies of the genera Drosophila (Diptera: Drosophilidae) and Neogriphoneura (Diptera: Lauxaniidae). Cells are generally rod-shaped, mesophilic, and measure 0.8-2.0 µm long by 0.3-0.5 µm wide. Optimal growth was observed under ambient atmosphere. Reconstruction of phylogenies from the 16S rRNA gene and from single-copy orthologs verify placement of these strains within Orbaceae. Cells exhibited similar fatty acid profiles to those of other <

SUBMITTER: Phillips LE 

PROVIDER: S-EPMC11434166 | biostudies-literature | 2024 Sep

REPOSITORIES: biostudies-literature