VEGF-FGF Signaling Activates Quiescent CD63⁺ Liver Stem Cells to Proliferate and Differentiate.

ABSTRACT: Understanding the liver stem cells (LSCs) holds great promise for new insights into liver diseases and liver regeneration. However, the heterogenicity and plasticity of liver cells have made it controversial. Here, by employing single-cell RNA-sequencing technology, transcriptome features of Krt19⁺ bile duct lineage cells isolated from Krt19CreERT; Rosa26R-GFP reporter mouse livers are examined. Distinct biliary epithelial cells which include adult LSCs, as well as their downstream hepatocytes and cholangiocytes are identified. Importantly, a novel cell surface LSCs marker, CD63, as well as CD56, which distinguished active and quiescent LSCs are discovered. Cell expansion and bi-potential differentiation in culture demonstrate the stemness ability of CD63⁺ cells in vi

SUBMITTER: Chen F 

PROVIDER: S-EPMC11434209 | biostudies-literature | 2024 Sep

REPOSITORIES: biostudies-literature