Identification of an Endogenous Strong Promoter in Burkholderia sp. JP2-270.

ABSTRACT: Burkholderia is the second largest source of natural product bacteria after Actinomyces and can produce many secondary metabolites including pyrrolnitrin (PRN). Natural products of microbial origin are usually found in trace amounts, so in metabolic engineering, promoter engineering is often used to regulate gene expression to increase yield. In this study, an endogenous strong promoter was identified based on RNA-seq to overexpress biosynthetic genes to increase the production of PRN. By analyzing the transcriptomic data of the antagonistic bacterium Burkholderia sp. JP2-270 in three different development periods, we screened 50 endogenous promoters with high transcriptional activity, nine of which were verified by an obvious fluorescent signal via fluorescence observ

SUBMITTER: Ke J 

PROVIDER: S-EPMC11434214 | biostudies-literature | 2024 Sep

REPOSITORIES: biostudies-literature