AraC Functional Suppressors of Mutations in the C-Terminal Domain of the RpoA Subunit of the Escherichia coli RNA Polymerase.

ABSTRACT: In E. coli, transcriptional activation is often mediated by the C-terminal domain of RpoA, the α subunit of RNA polymerase. Random mutations that prevent activation of the arabinose P_BAD promoter are clustered in the RpoA C-terminal domain (α-CTD). We have isolated functional suppressors of rpoA α-CTD mutations that map to araC, the main transcriptional regulator of ara genes, or to the P_BAD promoter. No mutation was found in the DNA regulatory region between araC and P_BAD. Most suppressors that improve P_BAD transcription are localized to the N-terminal domain of AraC. One class of araC mutations generates substitutions in the core of the N-terminal domain, suggesting that they affect its conformation. Ot

SUBMITTER: Belin D 

PROVIDER: S-EPMC11434276 | biostudies-literature | 2024 Sep

REPOSITORIES: biostudies-literature