Human induced pluripotent stem cell-derived cardiomyocytes to study inflammation-induced aberrant calcium transient.

ABSTRACT: Heart failure with preserved ejection fraction (HFpEF) is commonly found in persons living with HIV (PLWH) even when antiretroviral therapy suppresses HIV viremia. However, studying this condition has been challenging because an appropriate animal model is not available. In this article, we studied calcium transient in human induced pluripotent stem cell-derived cardiomyocytes (hiPSC-CMs) in culture to simulate the cardiomyocyte relaxation defect noted in PLWH and HFpEF and assess whether various drugs have an effect. We show that treatment of hiPSC-CMs with inflammatory cytokines (such as interferon-γ or TNF-α) impairs their Ca²⁺ uptake into sarcoplasmic reticulum and that SGLT2 inhibitors, clinically proven as effective for HFpEF, reverse this effect. Additionally, treatment w

SUBMITTER: Tatekoshi Y 

PROVIDER: S-EPMC11434618 | biostudies-literature | 2024 Sep

REPOSITORIES: biostudies-literature