Merging multi-omics with proteome integral solubility alteration unveils antibiotic mode of action.

ABSTRACT: Antimicrobial resistance is responsible for an alarming number of deaths, estimated at 5 million per year. To combat priority pathogens, like Helicobacter pylori, the development of novel therapies is of utmost importance. Understanding the molecular alterations induced by medications is critical for the design of multi-targeting treatments capable of eradicating the infection and mitigating its pathogenicity. However, the application of bulk omics approaches for unraveling drug molecular mechanisms of action is limited by their inability to discriminate between target-specific modifications and off-target effects. This study introduces a multi-omics method to overcome the existing limitation. For the first time, the Proteome Integral Solubility Alteration (PISA) assay is utilized i

SUBMITTER: Maity R 

PROVIDER: S-EPMC11434622 | biostudies-literature | 2024 Sep

REPOSITORIES: biostudies-literature