Exploration of the Binding Site of Arachidonic Acid in gp63 of <i>Leishmania mexicana</i> and in Orthologous Proteins in Clinically Important Parasites.

ABSTRACT: Recently, we published that the monoclonal antibody (D12 mAb) recognizes gp63 of L. mexicana, and it is responsible for COX activity. This D12 mAb exhibited cross-reactivity with Trypanosoma cruzi, Entamoeba histolytica, Acanthamoeba castellanii, and Naegleria fowleri. COX activity assays performed in these parasites suggested the potential presence of such enzymatic activity. In our investigation, we confirmed that wild-type recombinant gp63 exhibits COX-like activity, in contrast to a mutated recombinant gp63 variant. Consequently, our objective was to identify sequences orthologous to gp63 and subsequently analyze the binding of arachidonic acid (AA) to the putative active sites of these proteins. Given the absence of a crystallized structure for this

SUBMITTER: Hernandez-Ramirez VI 

PROVIDER: S-EPMC11434690 | biostudies-literature | 2024 Aug

REPOSITORIES: biostudies-literature