Assessment of High-Resolution Melting Curve Analysis for <i>Leishmania</i> spp. Detection in Different Clinical Manifestations of Leishmaniasis in India.

ABSTRACT: The accurate diagnosis and identification of Leishmania species are crucial for the therapeutic selection and effective treatment of leishmaniasis. This study aims to develop and evaluate the use of high-resolution melting curve analysis (HRM)-PCR for Leishmania species identification causing visceral leishmaniasis (VL), post-kala-azar dermal leishmaniasis (PKDL) and cutaneous leishmaniasis (CL) in the Indian subcontinent. Two multi-copy targets (ITS-1 and 7SL-RNA genes) were selected, and an HRM-PCR assay was established using L. donovani, L. major, and L. tropica standard strain DNA. The assay was applied on 93 clinical samples with confirmed Leishmania infection, including VL (n = 30), PKDL (n = 50), and CL (n = 13) cases. T

SUBMITTER: Azam M 

PROVIDER: S-EPMC11435223 | biostudies-literature | 2024 Sep

REPOSITORIES: biostudies-literature